Related Experiment Video
Updated: May 16, 2026
12:11
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Avidin-biotin based electrochemical immunoassay for thyrotropin
1Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK.
Annals of Clinical Biochemistry
|November 1, 1993
Summary
A novel electrochemical enzyme immunoassay was developed for sensitive thyrotropin (TSH) detection. This method utilizes specific antibodies and an enzyme label for accurate measurement, offering a new diagnostic tool.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Immunotechnology
Background:
- Thyrotropin (TSH) is a crucial biomarker for thyroid function assessment.
- Existing TSH assays may have limitations in sensitivity or cost-effectiveness.
- Development of novel, sensitive immunoassays is essential for improved diagnostics.
Purpose of the Study:
- To develop and validate a two-site electrochemical enzyme immunoassay for thyrotropin (TSH).
- To establish a sensitive and reliable method for TSH quantification.
- To explore the utility of screen-printed carbon electrodes in immunoassay development.
Main Methods:
- A two-site immunoassay format using immobilized capture and biotinylated detection antibodies.
- Enzyme labeling with alkaline phosphatase and avidin.
- Electrochemical detection of the enzymatic product (1-naphthol) using screen-printed carbon electrodes.
- Utilized 1-naphthyl phosphate as the substrate.
Main Results:
- Successful development of a two-site electrochemical enzyme immunoassay for TSH.
- Demonstrated detection of TSH using immobilized antibodies and an enzyme-labeled secondary antibody.
- Quantification of TSH through electrochemical detection of enzymatic activity on disposable electrodes.
Conclusions:
- The developed electrochemical enzyme immunoassay provides a sensitive method for TSH detection.
- This assay format holds promise for thyroid function diagnostics.
- The use of screen-printed electrodes offers potential for cost-effective and disposable TSH testing.
Related Concept Videos
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:20Synthesis and Regulation of Thyroid Hormones
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...