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Avidin-biotin based electrochemical immunoassay for thyrotropin

D Athey1, M Ball, C J McNeil

  • 1Department of Clinical Biochemistry, Medical School, University of Newcastle Upon Tyne, UK.

Annals of Clinical Biochemistry
|November 1, 1993
PubMed

A two site electrochemical enzyme immunoassay for thyrotropin (TSH) has been developed. This assay is based on the use of an immobilized capture antibody and a biotinylated second antibody. Detection is achieved via avidin labelled with alkaline phosphatase. The substrate 1-naphthyl phosphate was used and the product 1-naphthol was detected at disposable screen-printed carbon 8-electrode combs using specially designed instrumentation.

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