[Pregnancy via spermatozoa aspirated from the ductus deferens and fertilization in vitro]

The possibility of aspirating sperm from the first segments of the seminal tract by microsurgical techniques has permitted in vitro fertilization of the oocytes of the partners of men with obstructive azoospermia and ejaculation disorders. Sperm can be aspirated from the epididymis by microsurgical techniques in those cases with deferent duct agenesis or other obstructive pathologies, or from the deferent duct if spermatogenesis is unimpaired and there is no epididymal obstruction, but spermatozoa do not reach the ejaculate. We previously reported a pregnancy achieved by in vitro fertilization using sperm aspirated from the epididymis and the present article reports the first pregnancy achieved in our country using sperm aspirated from the deferent duct of the spouse with aspermia of psychogenic origin. A micropipette was utilized to obtain the sample, which was prepared using the mini-Percoll method. A total of 10 million motile spermatozoa were obtained and 6 of the 15 oocytes aspirated from the spouse were fertilized. The 3 embryos transferred to the fallopian tube resulted in a twin pregnancy that was carried to term.