Analysis of sister chromatid exchange and chromosome replication kinetics using Brd-U-dye techniques

The basis of BrdU-dye techniques for detecting DNA synthesis in cytological preparations is reviewed. General procedural modifications are described for applying this approach for the study of chromosome replication kinetics and sister chromatid exchange formation. Implications of sister chromatid exchange analysis for detecting mutagen-carcinogens and for differentiating human chromosome fragility diseases predisposing to neoplasia are discussed.