Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.8K
Experimental RNAi02:15

Experimental RNAi

8.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.1K
Nucleic Acid Structure01:25

Nucleic Acid Structure

9.7K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
9.7K
RNA Interference01:23

RNA Interference

28.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.3K
Complementary DNA01:44

Complementary DNA

31.9K
Overview
31.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis and characterization of chimeric 2-5A-DNA oligonucleotides.

Current protocols in nucleic acid chemistry·2008
Same author

Lung function reference values in Iranian adolescents.

Eastern Mediterranean health journal = La revue de sante de la Mediterranee orientale = al-Majallah al-sihhiyah li-sharq al-mutawassit·2007
Same author

RSV infections: developments in the search for new drugs.

Drug news & perspectives·2003
Same author

2-5A-antisense chimeras: inhibitors of respiratory syncytial virus infection.

Current opinion in molecular therapeutics·2001
Same author

Accelerating RNA decay through intervention of RNase L: alternative synthesis of composite 2',5'-oligoadenylate-antisense.

Methods in enzymology·2001
Same author

Chemistry and biochemistry of 2',5'-oligoadenylate-based antisense strategy.

Current medicinal chemistry·2001

Related Experiment Video

Updated: Mar 1, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.0K

2',5'-Oligoadenylate:antisense chimeras--synthesis and properties

K Lesiak1, S Khamnei, P F Torrence

  • 1Section on Biomedical Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.

Bioconjugate Chemistry
|November 1, 1993
PubMed
Summary

We created a novel bioconjugate linking an antisense oligonucleotide with a translation inhibitor (2-5A). This chimera selectively modulates mRNA expression by activating RNase and binding nucleases.

More Related Videos

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.9K
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
13:47

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

20.0K

Related Experiment Videos

Last Updated: Mar 1, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.0K
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.9K
Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
13:47

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

20.0K

Area of Science:

  • Molecular Biology
  • Oligonucleotide Synthesis
  • Bioconjugation Chemistry

Background:

  • Antisense oligonucleotides (ASOs) are key tools for gene silencing.
  • 2',5'-Oligoadenylates (2-5A) are potent activators of RNase L, involved in antiviral and antiproliferative responses.
  • Modulating mRNA expression requires precise targeting and efficient delivery mechanisms.

Purpose of the Study:

  • To synthesize a novel bioconjugate combining an antisense sequence with a 2-5A analog.
  • To evaluate the functional properties of the synthesized 2-5A antisense chimera.
  • To establish a new method for selective mRNA expression modulation.

Main Methods:

  • Solid-phase synthesis using the phosphotriester method for DNA synthesis.
  • Utilizing 4-hydroxybutyl phosphate residues as linkers for bioconjugation.
  • Characterization of the chimera's ability to activate 2-5A-dependent RNase and hybridize to target RNA.

Main Results:

  • Successful synthesis of a 2-5A antisense chimera with (dT)20 and a 2-5A moiety.
  • The chimera retained the 2-5A's RNase activating ability and the oligo(dT)'s hybridization capacity.
  • The bioconjugate effectively bound to the 2-5A-dependent nuclease upon annealing to the target sequence.

Conclusions:

  • The synthesized 2-5A antisense chimera is a functional molecule with dual properties.
  • This methodology offers a novel approach for targeted mRNA expression modulation.
  • The bioconjugate design shows promise for developing new therapeutic strategies targeting gene expression.