Enhanced robotic dissolution system with concurrent off-line analysis

A Zymate II robotic dissolution system was modified by addition of a liquid chromatography (LC) system equipped with an Isco ISIS autosampler. Using the Concurrent EasyLab programming language, operation of this LC system was integrated into the main robotic dissolution procedure. This enabled simultaneous dissolution sampling and LC analysis of standard and pre-pulled sample solutions. The resulting dual-tasking system effects considerable efficiency of operation and results in significant time savings. Comparison of the robotic and non-robotic data indicate good correlation and statistically insignificant differences at the 95% confidence level. Validation studies confirm linearity and precision of the quantitative LC method, accuracy and precision of volume and temperature measurements and negligible vessel-to-vessel carryover.