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Relationships between cotransducible plasmids in Staphylococcus aureus

S Iordănescu

    Journal of Bacteriology
    |January 1, 1977
    PubMed
    Summary
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    Staphylococcus aureus strains can cotransduce multiple plasmids, even without an active recombination system. Cotransduced plasmids can interact, forming new plasmids and influencing each other's replication.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacterial Genetics

    Background:

    • Plasmids are extrachromosomal DNA elements crucial for bacterial adaptation and evolution.
    • Understanding plasmid transfer mechanisms, like cotransduction, is vital for microbial genetics.
    • The role of host recombination systems in plasmid stability and transfer requires further investigation.

    Purpose of the Study:

    • To investigate the frequency and mechanism of plasmid cotransduction in Staphylococcus aureus.
    • To analyze genetic interactions between cotransduced plasmids and the formation of novel plasmids.
    • To explore the influence of cotransduced plasmids on the replication of other plasmids.

    Main Methods:

    • Utilized a Staphylococcus aureus strain harboring five distinct, compatible plasmids as a donor.

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  • Performed cotransduction experiments using both RecA+ (recombination-proficient) and RecA- (recombination-deficient) donors.
  • Analyzed plasmids in cotransductant clones using genetic and molecular techniques.

    • Main Results:

    • Observed cotransduction of two to three plasmids simultaneously.
    • Demonstrated that an active host recombination system is not essential for plasmid cotransduction.
    • Identified genetic interactions between cotransduced plasmids, resulting in the emergence of new plasmids with altered properties.

    Conclusions:

    • Plasmid cotransduction in Staphylococcus aureus can occur efficiently, irrespective of the host's recombination status.
    • Cotransduced plasmids can undergo genetic interactions, leading to the generation of novel plasmid structures.
    • A hypothesis of transient, reversible plasmid association is proposed to explain observed phenomena.