Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An algorithm accounting for plating efficiency in estimating spontaneous mutation rates

M E Jones1

  • 1School of Medicine, Flinders University, Adelaide, Australia.

Computers in Biology and Medicine
|November 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microsurgical training pre-and post-COVID 19: Is there a re-learning curve and lessons for lockdown three.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2021
Same author

Collateral damage: the impact on outcomes from cancer surgery of the COVID-19 pandemic.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

Challenges in the diagnosis of leptospirosis outwith endemic settings: a Scottish single centre experience.

The journal of the Royal College of Physicians of Edinburgh·2018
Same author

Electron transport and room temperature single-electron charging in 10 nm scale PtC nanostructures formed by electron beam induced deposition.

Nanotechnology·2017
Same author

Maximizing length and safety in gracilis free flap dissection.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2016
Same author

The importance of bone shortening in digital replantation.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2016
Same journal

Rapid personalisation of cardiovascular models using invasively measured right ventricular pressure.

Computers in biology and medicine·2026
Same journal

Biologically inspired mechanisms for enhancing robustness in EEG signal modeling: Challenges, opportunities, and perspectives.

Computers in biology and medicine·2026
Same journal

Machine learning-based detection of missed inspiratory efforts using esophageal pressure during noisy pressure support ventilation.

Computers in biology and medicine·2026
Same journal

A computational model of chemically- and mechanically-induced thrombus formation in cerebral aneurysms.

Computers in biology and medicine·2026
Same journal

An improved catch fish optimization based deep learning model for Parkinson disease classification using EEG signal.

Computers in biology and medicine·2026
Same journal

Assessing the robustness of evaluation metrics for synthetic ECG signal quality.

Computers in biology and medicine·2026
See all related articles

This study presents an algorithm and table to accurately calculate mutation rates from Luria-Delbrück experiments, even with imperfect plating efficiency. This improves the reliability of mutation rate estimations in microbial genetics.

Area of Science:

  • Microbiology
  • Genetics
  • Biostatistics

Background:

  • The Luria-Delbrück experiment is a cornerstone for measuring spontaneous mutation rates.
  • Accurate estimation requires accounting for factors like plating efficiency, which is often below 100%.

Purpose of the Study:

  • To develop a method for calculating mutation rates in Luria-Delbrück experiments with sub-optimal plating efficiency.
  • To provide a practical tool (a table) for researchers to estimate spontaneous mutation rates accurately.

Main Methods:

  • An algorithm was developed to model the probability distribution of mutant colonies.
  • The algorithm incorporates plating efficiency less than 100%.

Main Results:

  • The algorithm provides a precise method for calculating mutation rates under realistic experimental conditions.

Related Experiment Videos

  • A table is presented to simplify the estimation process, integrating plating efficiency.

    • Conclusions:

    • The developed algorithm and table enhance the accuracy of spontaneous mutation rate determination.
    • This work addresses a critical limitation in traditional Luria-Delbrück experiment analysis, improving data interpretation.