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Comparison of different ribozymes for efficient and specific cleavage of BCR/ABL related mRNAs
W Lange1, M Daskalakis, J Finke
1Universitätsklinik Freiburg, Abt. Hämatologie und Onkologie, Germany.
Abstract:
In chronic myelogenous leukemia (CML) the reciprocal translocation of the long arms of chromosomes results in the formation of the unique BCR/ABL fusion gene which is believed to play a crucial role in the pathogenesis of CML. Different short synthetic ribozyme constructs were compared with regard to their efficiency to cleave the BCR/ABL target RNA. In the CML cell line K562 we were able to inhibit the p210BCR/ABL synthesis by a ribozyme which was about twofold more effective than the corresponding antisense molecule.
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