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Effects of diatrizoate and iopamidol on spermatogenesis
V Yaghmai1, R S Harapanhalli, Y D Patel
1Department of Radiology, UMDNJ-New Jersey Medical School, Newark 07103.
Rationale And Objectives:
The biological effects of iodinated contrast media were examined by using spermatogenesis in mouse testis as the experimental model.
Methods:
Spermhead survival and abnormality assays were used as the biological end points. Diatrizoate meglumine/diatrizoate sodium and iopamidol were administered intravenously at equal rates and concentrations. Testicular uptake and clearance of these contrast agents were examined by high-performance liquid chromatography techniques. Appropriate mannitol solutions were employed as osmolality controls.
Results:
Intravenous administration of the contrast agent or its respective mannitol control resulted in approximately a 30% decrease in spermhead count. A dose-related experiment with mannitol demonstrated that the spermhead count decreased rapidly until 600 mOsm/kg was reached, beyond which this decrease was minimal. Clearance of both contrast media was complete in approximately 4 hours. No significant increase in the induction of spermhead abnormalities was observed.
Conclusion:
Osmotic substances, such as iodinated contrast agents, affect the process of spermatogenesis.