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Related Experiment Videos

Mouse serum amyloid A protein (SAA5) structure and expression

M C de Beer1, M S Kindy, W S Lane

  • 1Department of Biochemistry, University of Kentucky Medical Center, Lexington 40536.

The Journal of Biological Chemistry
|February 11, 1994
PubMed
Summary

A new mouse serum amyloid A protein, SAA5, is a normal component of high-density lipoprotein (HDL). Unlike other SAA proteins, SAA5 is constitutively expressed and plays a role in HDL

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Immunology

Background:

  • Serum amyloid A (SAA) proteins are acute-phase reactants involved in inflammation.
  • High-density lipoprotein (HDL) plays a role in lipid metabolism and immune response.
  • The diversity within the SAA protein family and their roles in normal physiology are not fully understood.

Purpose of the Study:

  • To identify and characterize a novel mouse SAA protein, SAA5.
  • To investigate the expression pattern and regulation of SAA5.
  • To elucidate the role of SAA5 in normal and acute-phase conditions, particularly its association with HDL.

Main Methods:

  • Isolation and sequencing of a cDNA clone from a normal mouse liver library to determine SAA5 structure.
  • Analysis of SAA5 amino acid sequence identity with other mouse and human SAA proteins.

Related Experiment Videos

  • Quantification of SAA5 mRNA levels and assessment of its response to inflammatory stimuli.
  • Investigation of plasma SAA5 levels during acute-phase responses.

    • Main Results:

    • A novel mouse SAA protein, SAA5, was identified as a normal apolipoprotein component of non-acute-phase HDL.
    • SAA5 shares 48% amino acid identity with other mouse SAA proteins and 57% with human C-SAA.
    • SAA5 mRNA is larger than other SAA mRNAs and is constitutively expressed in the liver with a muted response to inflammation.
    • Plasma SAA5 levels exhibit a biphasic response during the acute phase, suggesting translational control or altered HDL association.

    Conclusions:

    • SAA5 represents a constitutively expressed SAA protein that contributes to the normal physiological function of HDL.
    • Inducible SAA proteins (SAA1, SAA2, SAA3) likely confer an altered functional role to HDL during inflammation.
    • The distinct expression and regulation of SAA5 highlight functional specialization within the SAA protein family.