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Isolation of CD3-, CD4-, CD8-, IL-2R+ thymocyte precursors by panning
M Small1, A S Majumdar, M Lieberman
1Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Journal of Immunological Methods
|January 3, 1994
Abstract:
We present a panning method for isolation of thymocytes that are CD3-, CD4-, CD8- and IL-2R+. These cells have been isolated by positive selection on dishes coated with 7D4 antibody followed by treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.