[Effects of norepinephrine and alpha-1 adrenergic blockers on cultured stromal cell from benign prostatic...

Specimens obtained by open prostatectomy from patients with benign prostatic hyperplasia were used for primary stromal cell culture. Cell were treated with norepinephrine and alpha-1 blocker, prazosin. The cell proliferation was measured by 3H-thymidine incorporation and cell number. The cellular hypertrophy was assessed by flow cytometric analysis. Norepinephrine did not stimulate cell proliferation and prazosin did not induce to inhibit cell growth. Prazosin also did not have any effect on the hypertrophic stromal cell stimulated by serum. Our results suggested that alpha-1 adrenoceptors had no relation to the cellular hypertrophy or hyperplasia in the prostate.