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Related Experiment Videos

Functional nicotinic receptor expression in mesodermal cells transfected with MyoD cDNA

M Quik1, R Odeh, J Philie

  • 1Department of Pharmacology, McGill University, Montreal, Quebec, Canada.

Neuroscience
|December 1, 1993
PubMed
Summary

MyoD gene expression promotes the development of functional nicotinic acetylcholine receptors in cells. These receptors bind alpha-bungarotoxin and mediate sodium uptake, confirming their biological activity.

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Area of Science:

  • Molecular Biology
  • Neuroscience
  • Cell Biology

Background:

  • Previous research indicated MyoD promotes nicotinic acetylcholine subunit gene expression.
  • The functional significance of MyoD-induced gene expression for receptor development was unclear.

Purpose of the Study:

  • To investigate if MyoD promotes the development of functional nicotinic acetylcholine receptors.
  • To characterize the binding and functional properties of these MyoD-induced receptors.

Main Methods:

  • Transfection of C3H 10T1/2 cells with MyoD cDNA.
  • Assessment of [125I]alpha-bungarotoxin binding to detect receptor presence.
  • Measurement of 22Na+ uptake to evaluate receptor-mediated functional response.

Main Results:

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  • MyoD transfection led to high-affinity, saturable binding of [125I]alpha-bungarotoxin.
  • Cells exhibited a functional response, with carbachol stimulating 22Na+ uptake.
  • The binding and functional response were specific to nicotinic receptor ligands.

Conclusions:

  • MyoD expression induces the formation of functional nicotinic acetylcholine receptors on the cell surface.
  • The observed functional response is directly mediated by these newly developed receptors.