Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diverse essential functions revealed by complementing yeast calmodulin mutants

Y Ohya1, D Botstein

  • 1Department of Genetics, Stanford University School of Medicine, CA 94305.

Science (New York, N.Y.)
|February 18, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevention of atopic dermatitis in high-risk neonates via different types of moisturizer application: A randomized, blinded, parallel, three-group, phase II trial (PAF study).

Journal of the European Academy of Dermatology and Venereology : JEADV·2023
Same author

Combination of heat-killed <i>Lactiplantibacillus plantarum</i> YIT 0132 (LP0132) and oral immunotherapy in cow's milk allergy: a randomised controlled trial.

Beneficial microbes·2023
Same author

Non-invasive transcriptomic analysis using mRNAs in skin surface lipids obtained from children with mild-to-moderate atopic dermatitis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

Prostaglandin D<sub>2</sub> metabolite is not a useful clinical indicator for assessing atopic dermatitis.

Clinical and experimental dermatology·2020
Same author

Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.

Science advances·2020
Same author

Having small-for-gestational-age infants was associated with maternal allergic features in the JECS birth cohort.

Allergy·2018
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
See all related articles

Investigating yeast calmodulin mutants revealed distinct roles for different protein regions in essential cell functions like actin organization and nuclear division. These findings highlight calmodulin

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Calmodulin is a vital cytoplasmic calcium-binding protein essential for eukaryotic cell growth.
  • The CMD1 gene encodes the single essential calmodulin in yeast.

Purpose of the Study:

  • To investigate the diverse essential functions of calmodulin by analyzing mutations in the CMD1 gene.
  • To determine the roles of specific phenylalanine residues in calmodulin's function and localization.

Main Methods:

  • Analysis of 14 temperature-sensitive yeast mutants with phenylalanine to alanine substitutions in the CMD1 gene.
  • Classification of mutations into four intragenic complementation groups based on functional defects.

Main Results:

Related Experiment Videos

  • Mutations exhibited distinct defects in actin organization, calmodulin localization, nuclear division, and bud emergence.
  • Phenylalanine residues in the amino-terminal half are crucial for calmodulin localization and nuclear division.
  • Phenylalanine residues in the carboxyl-terminal half are critical for actin organization and bud emergence.

    • Conclusions:

    • Different regions of calmodulin are responsible for distinct essential cellular processes.
    • Specific phenylalanine residues play critical roles in mediating these functions, providing insights into calmodulin's structure-function relationship.