Related Experiment Video
Updated: Aug 9, 2026

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Published on: March 14, 2011
Bovine natural killer activity against virally infected cells inhibited by monoclonal antibodies
1Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison 53706.
Abstract:
Forty-four monoclonal antibodies (mAbs) were evaluated for their ability to alter natural killer (NK) cell lysis of virally infected target cells. Six of the mAbs inhibited the lysis of the target cells, while one of the mAbs enhanced lysis. Four of the inhibitory mAbs, CACT26A, CACT16A, CACTB45A and MUC76A, had very marked activity. These mAbs with inhibitory or enhancing activity recognized (1) WC2 molecules (CACTB44A, CACT16A, CACT26A) present on putative NK cells, (2) molecules on granulocytes, monocytes, a subpopulation of lymphocytes (CACTB45A and TH2A), (3) CD11a (MUC76A), and a protein of CD3 (MM1A).
Related Concept Videos
Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Cells of the Innate Immune Response
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Cytotoxic T Cells-mediated Immune Response
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

