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A sensitive mouse lymph node assay with two application phases for detection of contact allergens
Y Ikarashi1, T Tsuchiya, A Nakamura
1Division of Medical Devices, National Institute of Hygienic Sciences, Tokyo, Japan.
Archives of Toxicology
|January 1, 1993
Summary
A new mouse model accurately identifies contact sensitizing chemicals by measuring lymph node activation. This predictive test offers objective, quantitative results for screening potential sensitizers.
Area of Science:
- Toxicology
- Immunology
- Dermatology
Background:
- Contact sensitization is a significant health concern.
- Accurate predictive tests are needed to identify sensitizing chemicals.
- Existing methods may have limitations in detecting weak or moderate sensitizers.
Purpose of the Study:
- To develop and validate a predictive mouse test for identifying contact sensitizing chemicals.
- To optimize experimental conditions for assessing lymph node cell responses.
- To evaluate the test's performance against known sensitizers and non-sensitizers.
Main Methods:
- BALB/c mice received intradermal injections of test chemicals with adjuvant.
- Mice were subsequently exposed topically to chemicals for three consecutive days.
- Lymph node weight, cell counts, and lymph node cell (LNC) proliferation were assessed.
Main Results:
- The combined intradermal and topical application enhanced lymph node cell proliferation compared to topical application alone.
- The assay successfully identified the sensitizing capacity of ten chemicals, including weak sensitizers missed by other assays.
- Sodium lauryl sulfate (SLS), a non-sensitizer, did not induce significant lymph node responses.
Conclusions:
- The developed mouse lymph node assay is a sensitive screening tool for weak to moderate contact sensitizers.
- The assay provides objective and quantitative endpoints.
- It is suitable for evaluating colored or irritant chemicals, offering advantages over existing methods.