[The recording of maximal Ca-ATPase activity in intact human erythrocytes by using a Ca-selective electrode]

The method for recording Ca-ATPase activity in intact erythrocytes was devised. The parameters of Ca2+ efflux, mediated by Ca-ATPase were investigated in Ca(2+)-loaded red blood cells, using Ca(2+)-selective electrode. The red blood cell calcium concentration was modified using the ionophore A23187 in Ca(2+) medium. Recording Ca efflux from RBC may be used as a routine method for the differential diagnosis of patients with essential hypertension and other forms of cell pathology.