Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene activation in response to neural induction

P A Krieg1, A Zorn, N Ovsenek

  • 1Department of Zoology, University of Texas at Austin 78712.

Cellular & Molecular Biology Research
|January 1, 1993
PubMed
Summary

Researchers studied the neural cell adhesion molecule (NCAM) gene

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defective B cell development in Snell dwarf (dw/dw) mice can be corrected by thyroxine treatment.

Journal of immunology (Baltimore, Md. : 1950)·1996
Same author

The carboxyl terminus of GLUT4 contains a serine-leucine-leucine sequence that functions as a potent internalization motif in Chinese hamster ovary cells.

The Journal of biological chemistry·1996
Same author

Safe feeding practices for infants and young children.

Journal of paediatrics and child health·1996
Same author

Clinical associations and time of onset of cerebral white matter damage in very preterm babies.

Archives of disease in childhood. Fetal and neonatal edition·1996
Same author

Frequency-specific auditory brainstem responses in adults with sensorineural hearing loss.

Audiology : official organ of the International Society of Audiology·1996
Same author

Gun acquisition and use by juvenile offenders.

JAMA·1996

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Neural induction is critical for early vertebrate development.
  • Neural cell adhesion molecule (NCAM) expression is a key marker of neural induction.
  • Understanding NCAM gene regulation provides insights into neural development.

Purpose of the Study:

  • To investigate the transcriptional regulation of the Xenopus laevis NCAM gene.
  • To identify regulatory elements controlling NCAM expression during neural induction.
  • To elucidate the role of specific DNA-binding proteins in NCAM gene activation.

Main Methods:

  • Microinjection of reporter gene constructs into fertilized frog eggs.
  • Analysis of Xenopus NCAM promoter activity in response to neural induction signals.
  • Purification of DNA-binding proteins interacting with conserved promoter regions (OZ element and N-box).

Main Results:

  • The Xenopus NCAM promoter responds to neural induction signals.
  • A conserved region of the NCAM promoter contains an enhancer (OZ element) and a silencer (N-box).
  • Specific DNA-binding proteins interacting with both the OZ enhancer and N-box silencer were identified.

Conclusions:

  • The identified regulatory elements and proteins play a role in controlling NCAM expression.
  • These findings contribute to a model of transcriptional regulation during neural induction.
  • The study highlights conserved regulatory mechanisms in neural development.

Related Experiment Videos