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[Antepartum diagnosis of pseudohypertrophic muscular dystrophy using 14 sets of primers]
Y Wu1
1Peking Union Medical College Hospital, Beijing.
We used fourteen sets of primers to detect the gene deletion of 27 clinically diagnosed DMD patients by multiplex PCR technic. The detection rate was 59%. We found that the deletion region was more concentrated in the region of exon 43 to 52. The data was discussed. Twenty-two mothers of the twenty-seven affected children were pregnant again, and prenatal diagnosis was done by multiplex PCR of the DNA of the chorionic villi. Four affected fetuses were found and aborted.
We used fourteen sets of primers to detect the gene deletion of 27 clinically diagnosed DMD patients by multiplex PCR technic. The detection rate was 59%. We found that the deletion region was more concentrated in the region of exon 43 to 52. The data was discussed. Twenty-two mothers of the twenty-seven affected children were pregnant again, and prenatal diagnosis was done by multiplex PCR of the DNA of the chorionic villi. Four affected fetuses were found and aborted.