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Related Experiment Videos

Stimulation of pS2 expression by diet-derived compounds

N Sathyamoorthy1, T T Wang, J M Phang

  • 1Laboratory of Nutritional and Molecular Regulation, National Cancer Institute, NIH, Frederick, Maryland 21702-1201.

Cancer Research
|February 15, 1994
PubMed
Summary

Vegetarian diets may lower cancer risk due to plant compounds acting like estrogen. Researchers screened phenolic compounds, finding several with estrogen-like activity in breast cancer cells, suggesting a dietary cancer prevention mechanism.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nutritional Science

Background:

  • Epidemiological studies link vegetarian diets to reduced hormone-dependent cancer risk.
  • The underlying biological mechanisms for this association are not fully understood.
  • Dietary plant compounds like lignans and isoflavones can be converted into hormone-like substances by gut bacteria.

Purpose of the Study:

  • To investigate the potential of dietary compounds to mimic estrogenic activity.
  • To explore a novel, diet-dependent mechanism for cancer prevention.
  • To develop and utilize a cell-based assay to screen for estrogen-like compounds.

Main Methods:

  • Developed a specific assay system using estrogen receptor-positive MCF-7 breast cancer cells.
  • Monitored the expression of the estrogen-responsive protein pS2 via Northern blot analysis.

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  • Screened various phenolic compounds for estrogen-like activity in tissue culture.

    • Main Results:

    • Several phenolic compounds, including daidzein, equol, nordihydroguaiaretic acid, enterolactone, and kaempferol, demonstrated estrogen-like activity.
    • These compounds successfully elicited a response in the MCF-7 cell assay.
    • Quercetin and enterodiol did not exhibit significant estrogen-like activity in this system.

    Conclusions:

    • Certain dietary phenolic compounds possess estrogen-like activity, potentially explaining the reduced cancer risk observed in vegetarians.
    • This estrogenic activity, mediated by intestinal flora and acting on specific cellular pathways, represents a potential diet-dependent cancer prevention strategy.
    • The developed assay system is effective for screening compounds with estrogen-like properties relevant to hormone-dependent cancers.