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Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Cell Polarization by Rho Proteins

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

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Related Experiment Video

Updated: Jun 19, 2026

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Published on: August 27, 2011

Drosophila hedgehog acts as a morphogen in cellular patterning

J Heemskerk¹, S DiNardo

¹Department of Physiology, Columbia University, New York, New York 10032.

|February 11, 1994

Summary

This summary is machine-generated.

Engrailed-expressing cells in Drosophila embryos act as organizers, controlling cell fate through the hedgehog (hh) gene. Modulating hh levels suggests it functions as a concentration-dependent morphogen.

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Last Updated: Jun 19, 2026

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Published on: August 27, 2011

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

Published on: September 20, 2014

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

Area of Science:

Developmental biology
Genetics
Molecular biology

Background:

Embryogenesis relies on organizer regions to pattern cell types.
The precise mechanisms of segmental patterning in Drosophila are complex.

Purpose of the Study:

To investigate the organizer properties of engrailed-expressing cells in the Drosophila epidermis.
To elucidate the role of the hedgehog (hh) gene in mediating cell type differentiation during embryogenesis.

Main Methods:

Analysis of engrailed-expressing cells in Drosophila epidermis.
Modulation of hedgehog (hh) gene expression levels in embryos.
Modeling the interplay between hh and wingless signaling pathways.

Main Results:

Engrailed-expressing cells exhibit organizer properties, influencing epidermal cell differentiation.
The hedgehog (hh) gene mediates this patterning function.
Hedgehog (hh) acts as a morphogen, specifying cell fates in a concentration-dependent manner.

Conclusions:

Engrailed-expressing cells are key organizers in Drosophila epidermis.
Hedgehog (hh) signaling is crucial for establishing segmental cell type diversity.
A model integrating hh and wingless signaling explains segmental patterning.