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The human keratinocyte two-dimensional gel protein database: update 1993
J E Celis1, H H Rasmussen, E Olsen
1Institute of Medical Biochemistry, Aarhus University.
Electrophoresis
|November 1, 1993
Summary
This study details a comprehensive database of human keratinocyte proteins, identifying over 700 and cataloging thousands more. This resource aids in understanding skin disease mechanisms and gene expression patterns.
Area of Science:
- Proteomics
- Human Keratinocyte Biology
- Biochemical Databases
Background:
- Human keratinocytes are crucial for skin structure and function.
- Understanding keratinocyte protein expression is vital for diagnosing and treating skin diseases.
- Existing databases lack comprehensive proteomic data for human keratinocytes.
Purpose of the Study:
- To create a master database of human keratinocyte proteins.
- To identify and characterize proteins, including post-translational modifications.
- To facilitate the identification of abnormal gene expression and affected pathways in skin diseases.
Main Methods:
- Two-dimensional gel electrophoresis (isoelectric focusing and nonequilibrium pH gradient electrophoresis).
- Protein identification through microsequencing and database compilation.
- Bioinformatic analysis and visualization of protein data.
Main Results:
- The database currently lists 3038 cellular proteins from human keratinocytes.
- 763 proteins have been identified with detailed information (M(r), pI, localization).
- 176 microsequenced proteins are included, alongside curated sets of functional protein groups.
Conclusions:
- The established database provides a valuable resource for keratinocyte research.
- It enables the identification of altered protein expression patterns in skin conditions.
- This resource can help pinpoint signaling pathways involved in skin diseases, including cancer.