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Human liver protein map: update 1993

G J Hughes1, S Frutiger, N Paquet

  • 1Medical Biochemistry Department, Geneva University.

Electrophoresis
|November 1, 1993
PubMed
Summary
This summary is machine-generated.

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This study updates the human liver protein map, identifying 34 new polypeptide chains. These proteins are crucial for major liver biochemical pathways and are now cataloged in the 1993 SWISS-2DPAGE database.

Area of Science:

  • Biochemistry
  • Proteomics
  • Molecular Biology

Background:

  • The human liver proteome is essential for understanding hepatic function and disease.
  • Previous protein maps provided a foundation but required updates with advancements in identification techniques.

Purpose of the Study:

  • To update and expand the reference map of the human liver proteome.
  • To identify and characterize novel polypeptide chains within the human liver.
  • To integrate new findings into the SWISS-2DPAGE database for broader accessibility.

Main Methods:

  • Microsequencing techniques were employed to analyze protein spots from the human liver.
  • Silver staining was used for visualization and quantification of polypeptides on the 2D-PAGE map.
  • Protein identification involved comparing sequences against established protein databases.

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Main Results:

  • The updated human liver protein map includes 27 distinct spots, corresponding to 34 identified polypeptide chains.
  • The most abundant polypeptides identified are integral components of key hepatic biochemical pathways.
  • Newly identified proteins and previously known ones are systematically cataloged and labeled on the map.

Conclusions:

  • The 1993 reference human liver SWISS-2DPAGE database provides a comprehensive resource for hepatic proteomics.
  • The integration with the SWISS-PROT database facilitates cross-referencing and data retrieval.
  • This updated map enhances the understanding of liver protein composition and function.