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Plasma and red blood cell protein maps: update 1993
O Golaz1, G J Hughes, S Frutiger
1Medicine Department, Geneva University.
Electrophoresis
|November 1, 1993
Summary
This study updates the human plasma and red blood cell protein maps using advanced gel electrophoresis techniques. The findings provide a 1993 reference for the SWISS-2DPAGE database, aiding protein research.
Area of Science:
- Biochemistry
- Proteomics
Background:
- Two-dimensional gel electrophoresis is crucial for protein separation and analysis.
- Comprehensive protein databases are essential for biological research.
Purpose of the Study:
- To update the reference protein maps for human plasma and red blood cells.
- To provide the 1993 version of the SWISS-2DPAGE database.
Main Methods:
- Utilized immobilized pH gradients for high-resolution two-dimensional gel electrophoresis.
- Performed N-terminal sequencing and peptide mapping for protein characterization.
Main Results:
- Seventeen polypeptide spots/chains were partially characterized.
- Updated protein maps include new and known proteins for human plasma and red blood cells.
- The SWISS-2DPAGE database was updated for 1993.
Conclusions:
- The updated protein maps and database enhance the characterization of human plasma and red blood cell proteomes.
- Integration with SWISS-PROT database through common accession numbers facilitates further research.