Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Distinct structural elements of rab5 define its functional specificity

H Stenmark1, A Valencia, O Martinez

  • 1European Molecular Biology Laboratory, D-6900 Heidelberg, Germany.

The EMBO Journal
|February 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatin network markers of leukemia.

Bioinformatics (Oxford, England)·2020
Same author

Neoantigen prediction and computational perspectives towards clinical benefit: recommendations from the ESMO Precision Medicine Working Group.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

A systematic review and trial sequential analysis of intravenous vs. oral peri-operative paracetamol.

Anaesthesia·2020
Same author

Development of pipette tip-based poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolith for the extraction of drugs of abuse from oral fluid samples.

Talanta·2019
Same author

Women's interest in a personal breast cancer risk assessment and lifestyle advice at NHS mammography screening.

Journal of public health (Oxford, England)·2016
Same author

Assessment of stigma associated with tuberculosis in Mexico.

Public health action·2015
Same journal

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026
Same journal

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same journal

From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

The EMBO journal·2026
Same journal

Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

The EMBO journal·2026
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
See all related articles

Rab GTPases regulate vesicle transport. Researchers identified key structural regions in rab5 essential for its function in endocytosis and localization, demonstrating how specific elements dictate protein activity.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Structure-Function

Background:

  • Rab GTPases are crucial regulators of intracellular vesicle transport.
  • Rab5 specifically localizes to early endosomes and the plasma membrane, controlling endocytosis.
  • Understanding the structural basis of rab GTPase specificity is vital for deciphering cellular transport mechanisms.

Purpose of the Study:

  • To identify and characterize the structural elements responsible for rab5's specific localization and function in endocytosis.
  • To experimentally test the role of identified structural elements by creating chimeric rab proteins.

Main Methods:

  • Sequence alignment and molecular modeling to predict key structural regions of rab5.
  • Construction of chimeric rab proteins by transplanting structural elements between rab5 and rab6.

Related Experiment Videos

  • Assay of chimeric proteins for intracellular localization and their effect on endocytosis rates.

    • Main Results:

    • The C-terminus of rab5 could direct rab6 to the plasma membrane and early endosomes but did not restore rab5's endocytic function.
    • The N-terminus, helix alpha 2/loop 5, and helix alpha 2/loop 7 of rab5 were all necessary to confer rab5-like function onto rab6.
    • Reciprocal chimeras showed that these identified regions of rab5 are essential for its activity, as their replacement with rab6 regions rendered them inactive.

    Conclusions:

    • Distinct structural elements within rab5 precisely define its cellular localization, membrane association, and regulatory function in endocytosis.
    • This study elucidates the structure-function relationship of rab GTPases, highlighting the modular nature of their specificity.