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Related Experiment Videos

Ginkgo biloba extract EGb 761 or trolox C prevent the ascorbic acid/Fe2+ induced decrease in synaptosomal membrane

C Ramassamy1, F Girbe, Y Christen

  • 1Unité de Neuropsychopharmacologie Expérimentale, U.R.A. 1170 du C.N.R.S., Faculté de Médecine & Pharmacie de Rouen, Saint-Etienne du Rouvray, France.

Free Radical Research Communications
|January 1, 1993
PubMed
Summary
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Ascorbic acid and iron reduce dopamine uptake by synaptosomes by decreasing membrane fluidity. Antioxidants like Ginkgo biloba extract and quercetin protect dopamine transporter function.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Synaptosomes are crucial for studying neurotransmitter uptake.
  • Dopamine transporter (DAT) function is vital for dopaminergic neurotransmission.
  • Oxidative stress can impair neuronal function.

Purpose of the Study:

  • To investigate the effect of ascorbic acid on dopamine uptake in striatal synaptosomes.
  • To explore the role of membrane fluidity in dopamine transporter activity.
  • To identify potential protective agents against ascorbic acid-induced damage.

Main Methods:

  • Preparation of synaptosomes from striata.
  • Measurement of 3H-dopamine uptake at 37°C.
  • Assessment of synaptosomal membrane fluidity using fluorescence polarization with 1,6-diphenyl-1,3,5-hexatriene.

Related Experiment Videos

  • Evaluation of the effects of ascorbic acid, Fe2+ ions, and various antioxidants.

    • Main Results:

    • Ascorbic acid significantly reduced 3H-dopamine uptake in a concentration-dependent manner.
    • This reduction was associated with decreased synaptosomal membrane fluidity.
    • Fe2+ ions potentiated the decrease in membrane fluidity.
    • Desferrioxamine, Ginkgo biloba extract (EGb 761), quercetin, and trolox C prevented the decrease in membrane fluidity and protected dopamine uptake.

    Conclusions:

    • Ascorbic acid, particularly in the presence of Fe2+, induces lipid peroxidation in neuronal membranes.
    • This peroxidation leads to decreased membrane fluidity, impairing dopamine transporter function.
    • Antioxidants demonstrate neuroprotective effects by preserving membrane integrity and dopamine uptake.