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NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2
H L Drwinga1, L H Toji, C H Kim
1NIGMS Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research, Camden, New Jersey 08103.
Genomics
|May 1, 1993
Summary
The NIGMS Human Genetic Mutant Cell Repository offers two human/rodent somatic cell hybrid mapping panels. These well-characterized resources aid the Human Genome Project by providing DNA from cells with specific human chromosomes.
Area of Science:
- Genetics
- Cell Biology
- Genomics
Background:
- The Human Genome Project requires precise tools for mapping human genes.
- Somatic cell hybrids are crucial for assigning genes to specific chromosomes.
- Well-characterized mapping panels facilitate genetic research.
Purpose of the Study:
- To describe the NIGMS Human Genetic Mutant Cell Repository's somatic cell hybrid mapping panels.
- To detail the origin, preparation, and characterization of these valuable genetic resources.
- To highlight their utility for the Human Genome Project.
Main Methods:
- Distribution of two human/rodent somatic cell hybrid mapping panels.
- Panel 1: DNA from 18 hybrids with 1-19 human chromosomes.
- Panel 2: DNA from hybrids with 1-2 human chromosomes, available as DNA or cell cultures.
Main Results:
- Two distinct mapping panels are available for distribution.
- Panel 1 and Panel 2 contain hybrids retaining specific sets of human chromosomes.
- The majority of hybrids in Panel 2 retain a single intact human chromosome.
Conclusions:
- The described mapping panels are valuable resources for genetic research.
- These panels significantly support the goals of the Human Genome Project.
- The repository provides essential tools for human gene mapping and functional studies.