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Decrease in cellular replicative potential in "giant" mice transfected with the bovine growth hormone gene correlates

W R Pendergrass1, Y Li, D Jiang

  • 1Department of Pathology, University of Washington, Seattle 98195.

Journal of Cellular Physiology
|July 1, 1993
PubMed
Summary

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Mice engineered with the bovine growth hormone gene (bGH) grew larger but lived shorter lives. Their cells showed reduced ability to replicate, indicating a link between accelerated growth and cellular aging.

Area of Science:

  • Genetics and Molecular Biology
  • Cell Biology
  • Aging Research

Background:

  • Transgenic animals offer models for studying gene function.
  • Growth hormone influences growth and metabolism.
  • Accelerated growth may impact lifespan and cellular senescence.

Purpose of the Study:

  • To investigate the long-term effects of bovine growth hormone (bGH) gene expression on cellular replicative potential in mice.
  • To determine if accelerated growth due to bGH affects cellular aging.
  • To correlate cellular changes with observed lifespan reduction.

Main Methods:

  • Generation of transgenic mice with the bGH gene.
  • In vitro cell culture and clone size distribution analysis of fibroblasts and epithelial cells from various tissues.

Related Experiment Videos

  • Comparison of replicative potential between bGH+ mice and age-matched controls.

    • Main Results:

    • bGH+ mice exhibited significantly reduced mean lifespan compared to controls.
    • Cells from multiple tissues of bGH+ mice showed a decreased capacity for clonal expansion (reduced replicative potential).
    • Loss of replicative potential correlated with the period of accelerated growth (3-12 weeks) and increased GH receptor expression.

    Conclusions:

    • Transfection with the bGH gene leads to a trade-off between accelerated growth and cellular aging, evidenced by reduced replicative potential.
    • Accelerated growth, driven by bGH, may prime cells for senescence.
    • Cellular aging mechanisms are influenced by growth hormone signaling pathways.