Quantitative assessment of the astrocytic response in natural scrapie of sheep

Scrapie of sheep and goats belongs to the spongiform encephalopathies, a term derived from the characteristic vacuolar degeneration in the central nervous system. Astrocytosis has been described, but a systematic quantitative study has not been made. Such a study is important in resolving the still controversial issues as to whether the astrocytic response is caused directly by the infectious agent or represents a secondary reaction to tissue damage. In this study the numbers of astrocytes in 12 sheep with scrapie and 12 healthy sheep of a similar age were assessed and compared. Sections from eight planes of the brain were immunostained with anti-glial fibrillary acidic protein (GFAP) for astrocytes and their numbers counted in 40 areas of 1 mm2. The main findings were as follows. (1) A significant increase of astrocytes was detected in sheep with scrapie. (2) Astrocytosis was not usually related to the severity of the characteristic vacuolar lesions, indicating that it was at least partly due to a direct effect of the infectious agent but was not a secondary response to the neuronal damage. (3) The astrocytic response varied considerably between individual affected sheep; this may have been due to differences in agent strains, host response, or both.