Detection of Aeromonas hydrophila serogroup 0:34 in faeces using an enzyme-linked immunosorbent assay

A microtitration plate, antibody capture, enzyme-linked immunosorbent assay was developed for detection of Aeromonas hydrophila serogroup 0:34. The assay uses a detector antibody which shows no cross-reactions with Aeromonas strains not belonging to serogroup 0:34 or non-Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h; it is then microcentrifuged and the supernatant (unabsorbed antibody) titered on a microtiter plate coated with A. hydrophila cells from serogroup 0:34. All A. hydrophila strains from serogroup 0:34 that we tested in this manner reacted strongly with the detector antibody. Also, by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila 0:34 on different samples.