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Calcospherites in rabbit incisor predentin

H Mishima¹, Y Kunuki, T Sakae

¹Department of Anatomy, Nihon University School of Dentistry, Chiba, Japan.

Scanning Microscopy

|March 1, 1993

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Rabbit incisor dentin contains calcospherites, which are apatite crystals. Their size, shape, and collagen fibril content vary across the tooth, offering insights into dentin formation.

Area of Science:

Mineralized tissue biology
Dental research
Biomineralization

Background:

Calcospherites are mineralized globules found in dentin.
Their formation and composition are crucial for understanding dentin structure and properties.
Rabbit incisors provide a model system for studying dentin development.

Purpose of the Study:

To characterize the morphology, size, and elemental composition of calcospherites in rabbit lower incisor dentin.
To investigate the presence and arrangement of collagen fibrils within calcospherites.
To determine the crystalline structure and orientation of calcospherite minerals.

Main Methods:

Scanning and transmission electron microscopy (TEM) for morphological analysis.
Energy dispersive spectroscopy (EDS) for elemental composition analysis.

Related Experiment Videos

Electron diffraction for crystalline structure and orientation determination.

Main Results:

Calcospherites varied in size (0.3-31 microns) and shape (globular, mulberry, spindle) across different tooth regions.
Globular calcospherites in labial predentin contained collagen fibrils (120-170 nm width).
Crystals were identified as apatite, with preferred c-axis orientation in intermediate regions. Ca and P were major elements, with decreasing Mg/Ca ratios from apex to incisal horn.

Conclusions:

Calcospherite characteristics differ significantly between labial and lingual predentin and along the incisal-apical axis.
Collagen fibrils are associated with globular calcospherites in labial predentin, suggesting a role in matrix-mineralization.
Apatite composition and orientation indicate controlled biomineralization processes in rabbit incisor dentin.