The effect of vitamin A deficiency on testicular transfer RNA methyltransferase activity

Testicular transfer RNA methyltransferase activity was examined in normal and vitamin A-deficient rats. The specific activity was reduced by 50% in the vitamin A-deficient rats. In addition, a 5-fold decrease in the extent of tRNA methylation was observed with enzyme preparations from deficient testes. Both the rate and extent of tRNA methylation returned to control levels in vitamin A-repleted rats. In contrast, retinoic acid repletion did not reverse the effect of vitamin A deficiency on testicular tRNA methyltransferase activity. The methylated nucleoside composition of tRNA methylated by extracts of vitamin A-deficient testes was altered dramatically compared to that of tRNA methylated by control testicular enzymes. Decreased testicular tRNA methyltransferase activity was noted in midly deficient rats before the onset of testicular degeneration suggesting that the decreased tRNA methyltransferase activity in the testes is primarily the result of vitamin A deficiency.