Related Experiment Video
Updated: Jul 17, 2026
08:54
Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
Sugar transport in Saccharomyces cerevisiae
1Instituto de Investigaciones Biomédicas, CSIC, Madrid, Spain.
FEMS Microbiology Reviews
|April 1, 1993
Summary
This review details sugar transport in Saccharomyces cerevisiae, identifying specific transporters for monosaccharides like glucose and galactose, and disaccharides. It highlights how yeast regulates sugar uptake through transporter affinity and inactivation.
Area of Science:
- Molecular Biology
- Biochemistry
- Yeast Genetics
Background:
- Saccharomyces cerevisiae preferentially utilizes mono- and disaccharides as carbon sources.
- Cellular uptake of sugars necessitates specific membrane transport proteins.
- Understanding sugar transporters is crucial for yeast metabolism research.
Purpose of the Study:
- To review current knowledge on sugar transport mechanisms in Saccharomyces cerevisiae.
- To summarize identified transporters for monosaccharides and disaccharides.
- To discuss regulatory mechanisms influencing sugar uptake rates.
Main Methods:
- Kinetic analysis to identify transporter affinities and components.
- Gene identification (SNF3, HXT1, HXT2, GAL2) for specific transporters.
- Analysis of transporter expression regulation by carbon source and inducers/repressors.
Main Results:
- Two high- and low-affinity monosaccharide transporters identified, encoded by SNF3, HXT1, HXT2, and other uncharacterized genes.
- Galactose transport mediated by GAL2, induced by galactose and repressed by glucose.
- Maltose and alpha-methylglucoside transporters identified as H(+)-symports, regulated by inducer presence and glucose repression.
Conclusions:
- Multiple transporters exist for glucose, with varying affinities and regulatory controls.
- Galactose and disaccharide transport systems are well-defined but subject to glucose repression.
- Regulation of sugar uptake involves affinity modulation and Vmax changes, with underlying mechanisms requiring further investigation.
More Related Videos
07:38Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
06:53In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
Published on: February 21, 2025
Related Concept Videos
01:55Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
02:02Phloem and Sugar Transport
Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic cells that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic tissues to other specialized tissues by a process called translocation.
01:30Membrane Proteins
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
01:32Secondary Active Transport
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
01:27Glucose Transporters
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
01:26Glucose Absorption Into the Small Intestine
Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and 'exit' via the...