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Screening of synthetic peptide libraries with radiolabeled acceptor molecules
A Kassarjian1, V Schellenberger, C W Turck
1Howard Hughes Medical Institute, University of California, San Francisco.
Peptide Research
|May 1, 1993
Abstract:
A method has been developed for the identification of specific acceptor molecule-binding sequences from a chemically synthesized peptide library. The peptide resin beads, each bead carrying one peptide sequence, are incubated with radiolabeled acceptor molecule and subsequently immobilized in a thin layer of agarose. Resin beads that carry acceptor molecule-specific sequences are identified by autoradiography and subjected to automated gas-phase sequencing. The method was tested by screening a synthetic pentapeptide library with an anti-beta-endorphin monoclonal antibody.