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The development of an enzyme-linked immunosorbent assay for the determination of cortisol binding globulin
A W van Rijswijk1, J C Morgenthal, J J Taljaard
1Department of Human and Animal Physiology, University of Stellenbosch, South Africa.
Cell Biology International
|April 1, 1993
Abstract:
A simple, reliable and reproducible enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies for human cortisol binding globulin (CBG) has been developed. The sensitivity of the ELISA (1.20 fmol CBG/well) compared favourably with the sensitivity of other immunoassays. The excellent agreement (r = 0.98) seen between the present study and a binding assay indicates that the polyclonal antibodies used recognize only intact steroid-binding CBG. The intra- and inter-assay coefficient of variation (4.0% and 7.1% respectively) compared favourably with those reported by other authors.