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Related Experiment Videos

The International Standard for Thyroxine Binding Globulin

A F Bristow1, R E Gaines-Das, N Buttress

  • 1Division of Endocrinology, National Institute for Biological Standards and Control, Potters Bar, London, UK.

Clinical Endocrinology
|April 1, 1993
PubMed
Summary

A new international standard for thyroxine binding globulin (TBG) was established to ensure accurate thyroid hormone transport measurements. This highly pure TBG preparation, coded 88/638, provides a reliable reference for clinical diagnostics.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Endocrinology

Background:

  • Thyroxine binding globulin (TBG) is crucial for thyroid hormone transport in plasma.
  • Accurate quantitative estimation of TBG is clinically significant for diagnosing thyroid disorders.

Purpose of the Study:

  • To develop and validate a TBG preparation as an international standard.
  • To confirm its suitability through a multi-center collaborative study.
  • To assign a precise ampoule content for standardized use.

Main Methods:

  • Purification of TBG using affinity and conventional chromatography.
  • Characterization of purity and structure via gel electrophoresis, N-terminal sequencing, and mass spectroscopy.
  • Multi-center collaborative study to compare with local standards and assess stability.

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Main Results:

  • The TBG preparation (88/638) was confirmed to be >99% pure with a consistent structure.
  • Assays demonstrated consistent behavior compared to local standards.
  • Ampoule content was validated through physicochemical measurements and collaborative testing.

Conclusions:

  • WHO established the TBG preparation 88/638 as the International Standard for TBG.
  • The standard is assigned 30.0 International Units per ampoule, with 1 IU equivalent to 1 microgram.
  • This standard is available from the National Institute for Biological Standards and Control for global use.