Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse oocytes regulate granulosa cell steroidogenesis

B C Vanderhyden1, J N Cohen, P Morley

  • 1Department of Medicine, University of Ottawa, Ontario, Canada.

Endocrinology
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Novel Whole Tissue Explant Model of Hidradenitis Suppurativa.

bioRxiv : the preprint server for biology·2024
Same author

The Canadian Cancer Research Conference 2019.

Current oncology (Toronto, Ont.)·2020
Same author

Third International Havemeyer Workshop on Equine Herpesvirus type 1.

Equine veterinary journal·2012
Same author

A sero-survey of rinderpest in nomadic pastoral systems in central and southern Somalia from 2002 to 2003, using a spatially integrated random sampling approach.

Revue scientifique et technique (International Office of Epizootics)·2011
Same author

Evidence-based evolution of the high stakes postgraduate intensive care examination in Australia and New Zealand.

Anaesthesia and intensive care·2009
Same author

Anti-erythrocyte antibodies and disease associations in anemic and nonanemic dogs.

Journal of veterinary internal medicine·2008
Same journal

Loss of the calorie restriction response protein DEPP1 worsens diet-induced obesity.

Endocrinology·2026
Same journal

Long Non-Coding RNAs Mediate Endocrine Signaling and Resistance in Prostate Cancer.

Endocrinology·2026
Same journal

Repeated Chemogenetic Activation of C1 Catecholamine Neurons Reduces Subsequent Glucoprivic Responses and Mimics HAAF.

Endocrinology·2026
Same journal

Postnatal exposure to maternal hypothyroidism leads to developmental delay and metabolic dysregulations in male mice.

Endocrinology·2026
Same journal

Discovery proteomics identification of factors contributing to gonadotropin β expression.

Endocrinology·2026
Same journal

3D genome architecture and epigenetic regulation of lineage identity in advanced prostate cancer.

Endocrinology·2026
See all related articles

Oocytes regulate granulosa cell steroid production. Removing oocytes (oocytectomy) increased progesterone but decreased estradiol, suggesting oocyte factors modulate these key reproductive hormones.

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Cell Biology

Background:

  • Oocytes influence granulosa cell proliferation, differentiation, and expansion.
  • The role of oocytes in regulating granulosa cell steroidogenesis remains less understood.

Purpose of the Study:

  • To investigate whether oocytes modulate steroid production by cumulus granulosa cells.
  • To determine the specific effects of oocyte-derived factors on progesterone and estradiol synthesis.

Main Methods:

  • Culture of intact and oocytectomized (OOX) mouse oocyte-cumulus cell complexes.
  • Treatment with follicle-stimulating hormone (FSH) and testosterone (T).
  • Analysis of progesterone and estradiol production in culture supernatants.

Related Experiment Videos

Main Results:

  • Oocytectomy significantly increased progesterone production in response to FSH and FSH+T.
  • Oocyte-conditioned medium inhibited progesterone production by OOX complexes.
  • Oocytectomy slightly decreased estradiol production, but oocyte-conditioned medium stimulated it in OOX complexes.

Conclusions:

  • Mouse oocytes secrete factors that inhibit progesterone production by cumulus granulosa cells.
  • Oocytes also secrete factors that stimulate estradiol production by cumulus granulosa cells.
  • Oocyte-derived factors play a crucial role in regulating granulosa cell steroidogenesis.