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Related Experiment Videos

Urinary markers for exposures to alkylating or nitrosating agents

J S Wishnok1, S R Tannenbaum, W G Stillwell

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.

Environmental Health Perspectives
|March 1, 1993
PubMed
Summary

Urinary nitrosoproline and nitrate levels correlate with advanced gastric pathology in high-risk populations. New research explores nitric oxide (NO) biomarkers for endogenous nitrosation and gastric cancer etiology.

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Area of Science:

  • Biochemistry
  • Gastroenterology
  • Oncology

Background:

  • Endogenous nitrosation is implicated in gastric cancer etiology.
  • Urinary markers are investigated for assessing nitrosation and cancer risk.
  • Nitric oxide (NO) plays a role as a cellular messenger.

Purpose of the Study:

  • To investigate urinary markers for endogenous nitrosation and gastric cancer.
  • To explore nitric oxide (NO) related compounds as potential biomarkers.
  • To assess the relationship between urinary markers and gastric pathology in a high-risk population.

Main Methods:

  • Analysis of urinary nitrosoproline and nitrate excretion in a Colombian population.
  • Measurement of urinary 7-methylguanine and 3-methyladenine.

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  • Evaluation of nitrosohydroxyethylglycine and 3-nitrotyrosine as potential biomarkers in experimental models.

    • Main Results:

    • Urinary nitrosoproline correlated with nitrate in subjects with advanced gastric pathology.
    • No strong relationship was found between 7-methylguanine/3-methyladenine and gastric pathology or nitrosation markers.
    • Prolonged exercise and 15N-arginine ingestion increased urinary nitrate.
    • Nitrosohydroxyethylglycine increased in rats after morpholine administration and immunostimulation, but nitrotyrosine was not a useful biomarker.

    Conclusions:

    • Urinary nitrate and nitrosoproline may serve as indicators of endogenous nitrosation in gastric cancer patients.
    • Further research is needed to validate nitric oxide (NO) related biomarkers for human studies.
    • The study highlights the complexity of endogenous nitrosation pathways in gastric cancer development.