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The murine cot proto-oncogene: genome structure and tissue-specific expression

R Ohara¹, J Miyoshi, M Aoki

¹Department of Oncogene Research, Osaka University.

Japanese Journal of Cancer Research : Gann

|May 1, 1993

Summary

Researchers cloned and analyzed the murine cot proto-oncogene, finding its gene structure conserved with humans. The cot gene is expressed across various mouse tissues from fetal to adult stages, albeit at low levels.

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Area of Science:

Molecular Biology
Genetics
Oncogenes

Background:

The cot proto-oncogene plays a role in cellular processes.
Understanding the murine cot proto-oncogene is crucial for comparative genomics and cancer research.

Purpose of the Study:

To clone and characterize the murine cot proto-oncogene.
To investigate the tissue-specific expression patterns of the cot gene in mice.

Main Methods:

Gene cloning and sequencing of murine cot proto-oncogene.
Analysis of genomic DNA and cDNA sequences.
Northern blot analysis for gene expression studies.

Main Results:

Murine cot proto-oncogene comprises eight exons, with conserved intron-exon borders compared to the human homolog.

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Full-length cot cDNA has a 1,401-nucleotide open reading frame.

Sequence identity between murine and human cot genes is 84.4% at the nucleotide level and 93.9% at the amino acid level.

The cot gene message is approximately 2.9 kb and expressed in multiple tissues from fetal to adult mice, with low overall expression levels.

Conclusions:

The murine cot proto-oncogene shares significant structural and sequence homology with its human counterpart.
The cot gene exhibits a broad but low-level expression pattern across various tissues during mouse development and adulthood.