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Related Experiment Videos

Spreading depression in isolated carp retina

H Higashida, M Sakakibara, G Mitarai

    Brain Research
    |January 14, 1977
    PubMed
    Summary

    Spreading depression (SD) in carp retina shares similarities with other vertebrates. Low chloride solutions reduce the threshold for SD, impacting retinal cell activity and potentials.

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    Area of Science:

    • Neuroscience
    • Ophthalmology
    • Physiology

    Background:

    • Spreading depression (SD) is a wave of neuronal and glial depolarization.
    • Understanding SD in the retina is crucial for visual processing research.

    Purpose of the Study:

    • To investigate the characteristics of spreading depression (SD) in isolated carp retina.
    • To examine the effects of low chloride solutions on SD generation and properties.
    • To analyze the impact of SD on various retinal cell types and potentials.

    Main Methods:

    • Eliciting spreading depression (SD) in isolated carp retina using KCl application.
    • Immersion of retinal tissue in low chloride Ringer's solutions to alter SD threshold.
    • Recording extracellular potential shifts across retinal layers during SD.
    • Measuring PIII potential of the electroretinogram.
    • Evoking S-potentials in horizontal cells during SD.
    • Observing spike activity in on- and off-center ganglion cells.

    Main Results:

    • SD was successfully elicited in carp retina, showing similarities to other vertebrates.
    • Low chloride solutions significantly reduced the threshold for SD generation.
    • SD waves exhibited similar properties but altered intervals in treated retinas.
    • Extracellular negative potential shifts during SD averaged 4.6 mV in amplitude and 27 sec in duration.
    • PIII potential remained largely unaffected by SD.
    • Horizontal cells showed increased S-potential amplitude during SD-induced membrane depolarization.
    • Ganglion cells displayed altered spike activity, with decreased off-discharges in immersed retinas.

    Conclusions:

    • Carp retina exhibits spreading depression (SD) with conserved vertebrate characteristics.
    • Retinal SD can be modulated by ionic environment, specifically chloride concentration.
    • SD significantly impacts horizontal and ganglion cell activity, suggesting functional consequences for visual processing.

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