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Related Experiment Videos

Intravaginal immunization in sheep using a bioadhesive microsphere antigen delivery system

D T O'Hagan1, D Rafferty, S Wharton

  • 1Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.

Vaccine
|January 1, 1993
PubMed
Summary

Intravaginal immunization with influenza haemagglutinin (TOPS) and degradable starch microspheres/lysophosphatidylcholine (DSM/LPC) enhanced antibody responses in sheep. However, intramuscular immunization remained superior for inducing high antibody levels.

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Area of Science:

  • Immunology
  • Vaccinology
  • Biotechnology

Background:

  • Influenza virus haemagglutinin (TOPS) is a key antigen for vaccine development.
  • Intravaginal antigen delivery systems offer potential for mucosal immunization.
  • Lysophosphatidylcholine (LPC) and degradable starch microspheres (DSM) are components of novel drug delivery systems.

Purpose of the Study:

  • To evaluate an intravaginal antigen delivery system using TOPS, LPC, and DSM.
  • To compare the immunogenicity of intravaginal versus intramuscular immunization routes.
  • To assess serum and vaginal antibody responses (IgA and IgG) in sheep.

Main Methods:

  • Sheep were immunized intravaginally or intramuscularly with TOPS formulations.
  • Formulations included TOPS in solution, TOPS with DSM/LPC powder, and TOPS with LPC solution.

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  • Serum and vaginal wash samples were collected and analyzed for IgA and IgG antibody levels post-immunization.

    • Main Results:

    • Intravaginal immunization with TOPS and DSM/LPC resulted in significantly higher serum IgG and vaginal IgA responses compared to TOPS alone.
    • Intramuscular immunization with TOPS and aluminium hydroxide gel (Alugel) induced the highest antibody levels in both serum and vaginal washes.
    • Intravaginal immunization with TOPS and LPC alone did not enhance antibody levels.

    Conclusions:

    • The combination of TOPS with DSM/LPC shows promise as an effective intravaginal antigen delivery system.
    • Intramuscular immunization remains the most potent route for inducing systemic and mucosal antibody responses in this model.
    • Further research into optimizing intravaginal delivery systems for influenza antigens is warranted.