Inhibition of neutrophil chemotaxis by 4-hydroxycyclophosphamide

4-Hydroxycyclophosphamide, phosphoramide mustard, and nor-nitrogen mustard are compounds considered as candidates for the biologically active, alkylating metabolite of cyclophosphamide. These compounds were tested for inhibition of chemotaxis in neutrophils from normal human donors. Of those tested, only 4-hydroxycyclophosphamide caused a significant (p less than 0.01) decrease in the chemotactic index. The chemical precursor of 4-hydroxycyclophosphamide, 4-hydroperoxycyclophosphamide, caused a barely significant (p less than 0.05) decrease; 4-ketocyclophosphamide had no effect.