Related Experiment Videos
Dual marker one day pancreolauryl test
M R Green1, S Austin, L T Weaver
1MRC Dunn Nutrition Unit, Cambridge.
Archives of Disease in Childhood
|May 1, 1993
Summary
A new non-invasive test simplifies diagnosing exocrine pancreatic dysfunction in children. The modified pancreolauryl test uses fluorescein dilaurate and mannitol, offering clear discrimination between healthy children and cystic fibrosis patients in a single day.
Area of Science:
- Pediatric Gastroenterology
- Diagnostic Medicine
- Biochemistry
Background:
- Exocrine pancreatic dysfunction diagnosis in children requires simple, non-invasive methods.
- Current tests may involve multiple collections, complicating pediatric use.
Purpose of the Study:
- To develop and validate a single-day, non-invasive test for exocrine pancreatic function in children.
- To modify the pancreolauryl test with an additional marker for improved efficiency.
Main Methods:
- A modified pancreolauryl test was administered to six healthy subjects and nine cystic fibrosis patients.
- Subjects ingested fluorescein dilaurate and mannitol, with urine collected over 10 hours.
- Urinary fluorescein and mannitol levels were quantified spectrophotometrically and enzymatically, respectively.
Main Results:
- The ratio of fluorescein to mannitol recovery (F:M ratio) clearly distinguished healthy subjects from cystic fibrosis patients.
- This discrimination was evident regardless of enzyme replacement therapy in cystic fibrosis patients.
- Significant differences in F:M ratios were observed in urine collected 2–8 hours post-ingestion.
Conclusions:
- The modified pancreolauryl test, using fluorescein dilaurate and mannitol, is a simple, single-day, tubeless method for assessing exocrine pancreatic function.
- This test significantly simplifies the investigation of suspected exocrine pancreatic dysfunction in pediatric patients.