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Sequencing of a urinary stone protein, identical to alpha-one antitrypsin, which lacks 22 amino acids
T Umekawa1, K Kohri, N Amasaki
1Department of Urology, School of Medicine, Kinki University, Osaka, Japan.
Biochemical and Biophysical Research Communications
|June 30, 1993
Abstract:
We have extracted and purified proteins from calcium containing urinary stones with 4 M guanidine chloride and sequenced the amino acids of the proteins. The protein of approximately 49kDa was defined as human alpha-one antitrypsin (AT, from 23rd to 42nd amino acids of NH2-terminal) by amino acid sequence analysis. The ratio of the amount of AT to total proteins was 34.9% analyzed by densitometer. AT is presumably involved in stone formation as a coprecipitating substance because equilibrium dialysis revealed that AT has no affinity for calcium.