Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microring-ring electrode for manipulation of a single cell

T Matsue1, N Matsumoto, S Koike

  • 1Department of Molecular Chemistry and Engineering, Faculty of Engineering, Tohoku University, Sendai, Japan.

Biochimica Et Biophysica Acta
|July 11, 1993
PubMed
Summary

Dielectrophoretic manipulation successfully trapped a single myeloma cell using microring-ring electrodes. Intense electric fields generated by applied AC voltages drove the dielectrophoretic force for cell manipulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterisation of antibiotic resistance of Salmonella isolated from dog treats in Japan.

Epidemiology and infection·2019
Same author

Selection of macrolide-resistant Campylobacter in pigs treated with macrolides.

The Veterinary record·2014
Same author

Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy.

Analytical chemistry·2011
Same author

Nationwide surveillance of salmonella in the faeces of pigs in Japan.

Zoonoses and public health·2008
Same author

Bacteriological characteristics of Staphylococcus aureus isolates from humans and bulk milk.

Journal of dairy science·2008
Same author

Rapid and effective detection of anthrax spores in soil by PCR.

Journal of applied microbiology·2003

Area of Science:

  • Biophysics
  • Electrical Engineering
  • Cell Biology

Background:

  • Dielectrophoresis is a phenomenon used for manipulating biological cells.
  • Microring-ring electrodes offer precise control over electric fields.
  • Myeloma cells are a type of cancer cell relevant to biological studies.

Purpose of the Study:

  • To demonstrate the dielectrophoretic manipulation of a single myeloma cell.
  • To investigate the use of microring-ring electrodes for cell trapping.
  • To analyze the effect of applied AC voltages on cell movement.

Main Methods:

  • Utilized microring-ring electrodes with tip radii of 3-5 microns.
  • Applied alternating current (AC) voltages to generate electric fields.
  • Observed and recorded the movement and trapping of a single myeloma cell.

Related Experiment Videos

Main Results:

  • The single myeloma cell was successfully moved and trapped at the electrode tip.
  • Dielectrophoretic forces induced by locally intense electric fields were responsible for cell manipulation.
  • The applied AC voltages effectively controlled the cell's position.

Conclusions:

  • Microring-ring electrodes are effective tools for the dielectrophoretic manipulation of single myeloma cells.
  • Dielectrophoretic forces can precisely control cell positioning at the microscale.
  • This technique shows potential for applications in cell sorting and analysis.