Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Neutrophil-specific granule deficiency includes eosinophils

H F Rosenberg1, J I Gallin

  • 1Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Blood
|July 1, 1993
PubMed
Summary

Neutrophil-specific granule deficiency may affect eosinophils, revealing a unique cell population. These cells contain eosinophil peroxidase but lack key granule proteins, challenging previous understandings of leukocyte maturation disorders.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alternaria alternata challenge at the nasal mucosa results in eosinophilic inflammation and increased susceptibility to influenza virus infection.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2018
Same author

Eosinophil persistence in vivo and sustained viability ex vivo in response to respiratory challenge with fungal allergens.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2017
Same author

Nine patients with chronic granulomatous disease having selective neck dissection for severe cervical lymphadenitis.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2017
Same author

In Memory and Celebration: Dr. James J. Lee.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2017
Same author

Modulation of the inflammatory response by products released from human polymorphonuclear leukocytes during phagocytosis : Generation and inactivation of the chemotactic factor C5a.

Inflammation·2013
Same author

Inflammatory responses to respiratory syncytial virus (RSV) infection and the development of immunomodulatory pharmacotherapeutics.

Current medicinal chemistry·2012

Area of Science:

  • Hematology
  • Cell Biology
  • Immunology

Background:

  • Neutrophil-specific granule deficiency is a leukocyte disorder affecting neutrophil maturation.
  • It is characterized by reduced mRNA for specific granule proteins.
  • This disorder was previously believed to be confined to neutrophils.

Purpose of the Study:

  • To investigate if neutrophil-specific granule deficiency impacts other leukocyte lineages.
  • To characterize a newly identified population of peripheral white blood cells.

Main Methods:

  • Immunofluorescence staining to detect eosinophil peroxidase (EPO).
  • Light microscopy for cell morphology assessment.
  • Analysis of mRNA transcripts and protein content for specific granule proteins.

Related Experiment Videos

Main Results:

  • A distinct population of peripheral white blood cells expressing EPO (EPO+ cells) was identified.
  • These EPO+ cells lacked typical eosinophil granules and were morphologically similar to granule-deficient neutrophils.
  • EPO+ cells responded to granulocyte-macrophage colony-stimulating factor (GM-CSF) with a 70-fold increase in population size.
  • EPO+ cells contained Charcot-Leyden crystal protein but were deficient in eosinophil cationic protein, eosinophil-derived neurotoxin, and major basic protein, despite present mRNA for these proteins.

Conclusions:

  • Neutrophil-specific granule deficiency can extend to eosinophils, presenting as a unique EPO+ cell population.
  • These cells represent an aberrant eosinophil lineage with selective granule protein deficiency.
  • The findings expand the understanding of leukocyte maturation disorders and their impact on myeloid cell differentiation.