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Desmin sequence elements regulating skeletal muscle-specific expression in transgenic mice

Z Li1, P Marchand, J Humbert

  • 1Biologie Moléculaire de la Différenciation, Université Paris 7, France.

Development (Cambridge, England)
|March 1, 1993
PubMed
Summary
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Desmin gene regulatory elements direct muscle-specific expression in transgenic mice. This study reveals desmin sequences predetermine myogenesis in migrating cells and highlights expression in the heart

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Desmin is an early muscle protein in mouse embryos, with unique gene expression patterns.
  • Desmin expression begins in replicating myoblasts and increases during muscle differentiation.

Purpose of the Study:

  • To characterize a muscle-specific enhancer for the desmin gene.
  • To generate and analyze transgenic mice carrying a desmin promoter-reporter gene construct (Des1-nlacZ).
  • To investigate the regulatory elements controlling desmin expression during embryonic development.

Main Methods:

  • Generation of transgenic mice with a Des1-nlacZ transgene.
  • Analysis of reporter gene expression in tissue sections of transgenic embryos.
  • Comparison of transgene activity with endogenous desmin expression patterns.

Related Experiment Videos

Main Results:

  • The 5' regulatory sequence of the desmin gene (Des1-nlacZ) directs strong, muscle-specific expression in vitro and in vivo.
  • Transgene expression is developmentally regulated and correlates with endogenous desmin in skeletal muscle development.
  • Desmin sequences direct selective expression in cells migrating from somites to limb buds, indicating predetermination for myogenesis.
  • Smooth and cardiac muscle cells showed no reporter gene expression, except for the cardiac conduction system.

Conclusions:

  • The characterized desmin promoter elements are sufficient for muscle-specific and developmentally regulated gene expression.
  • Desmin regulatory sequences identify cells committed to myogenesis early in development, including migrating somite-derived cells.
  • The findings provide insights into the molecular mechanisms controlling skeletal muscle development and cell fate determination.