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Related Experiment Videos

A chromosome 11 YAC library

S Qin1, J Zhang, C M Isaacs

  • 1Department of Human Genetics, Roswell Park Cancer Institute, New York State Department of Health, Buffalo 14263.

Genomics
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

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A new yeast artificial chromosome (YAC) library for human chromosome 11 was created, offering extensive coverage and enabling efficient gene mapping. This resource facilitates the assembly of contiguous sequences for chromosome 11 research.

Area of Science:

  • Genomics
  • Molecular Biology
  • Human Genetics

Background:

  • Construction of targeted libraries is crucial for efficient genome mapping.
  • Previous methods faced challenges with interspecies chimeric clones and contig assembly.
  • A comprehensive resource for chromosome 11 is needed for detailed genetic studies.

Purpose of the Study:

  • To construct and characterize a targeted yeast artificial chromosome (YAC) library for human chromosome 11.
  • To assess the library's coverage, clone integrity, and utility for gene mapping and contig assembly.
  • To provide a valuable resource for researchers studying chromosome 11.

Main Methods:

  • Generation of a yeast artificial chromosome (YAC) library from the J1 cell line containing human chromosome 11.

Related Experiment Videos

  • Screening and elimination of interspecies chimeric clones.
  • Characterization of YAC inserts (average size 337 kb) and library coverage (fourfold).
  • Fluorescence in situ hybridization (FISH) for YAC clone localization.
  • Screening with specific chromosome 11 markers (HBB, GLUR4, H19, D11S193).

    • Main Results:

    • A library of over 1824 YAC clones was successfully constructed.
    • The library provides fourfold coverage of chromosome 11 with an average insert size of 337 kb.
    • YAC clones were randomly distributed and localized via FISH.
    • Specific YAC clones were isolated for chromosome 11 markers HBB, GLUR4, H19, and D11S193.
    • The library demonstrated an unbiased representation and feasibility for contig assembly.

    Conclusions:

    • The constructed YAC library is a high-quality, unbiased resource for chromosome 11 research.
    • The library facilitates efficient recovery of YAC clones and contig assembly.
    • This resource will significantly aid in the detailed mapping and analysis of human chromosome 11.