Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Adenovirus-E1A proteins transform cells by sequestering regulatory proteins

D S Peeper1, A Zantema

  • 1Lab. Molecular Carcinogenesis, Leiden, The Netherlands.

Molecular Biology Reports
|April 1, 1993
PubMed
Summary

Adenovirus E1A proteins transform cells by binding to cellular proteins like retinoblastoma (Rb). This interaction releases transcription factor E2F, driving cell transformation and linking E1A

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens.

Immuno-oncology technology·2022
Same author

Survival and biomarker analyses from the OpACIN-neo and OpACIN neoadjuvant immunotherapy trials in stage III melanoma.

Nature medicine·2021
Same author

Presence of a 34-gene signature is a favorable prognostic marker in squamous non-small cell lung carcinoma.

Journal of translational medicine·2020
Same author

Zeb1 is required for TrkB-induced epithelial-mesenchymal transition, anoikis resistance and metastasis.

Oncogene·2011
Same author

BRAF(E600) in benign and malignant human tumours.

Oncogene·2007
Same author

Oncogene-induced cell senescence--halting on the road to cancer.

The New England journal of medicine·2006

Area of Science:

  • Molecular Biology
  • Virology
  • Cancer Research

Background:

  • Adenovirus E1A proteins are key mediators of viral-induced cell transformation.
  • E1A protein function relies on interactions with various cellular proteins.
  • Understanding these interactions is crucial for deciphering oncogenic mechanisms.

Purpose of the Study:

  • To review the properties of E1A proteins concerning their cellular binding partners.
  • To elucidate the mechanism by which E1A proteins induce cell transformation.
  • To identify the cellular proteins that associate with E1A.

Main Methods:

  • Literature review of studies investigating E1A protein interactions.
  • Analysis of known cellular binding partners of E1A.

Related Experiment Videos

  • Examination of the functional consequences of E1A-protein binding.

    • Main Results:

    • Identified key E1A-binding cellular proteins: retinoblastoma-susceptibility protein (Rb), p107, cyclin A, and p33cdk2.
    • These proteins also bind to transcription factor E2F, with Rb binding repressing E2F's activity.
    • E1A sequesters regulatory proteins like Rb, liberating active E2F and promoting transcription.

    Conclusions:

    • E1A-mediated cell transformation is directly linked to the disruption of cellular protein functions.
    • The ability of E1A to bind Rb and release E2F is essential for its transforming properties.
    • Specific domains within E1A are critical for both transcriptional regulation and oncogenesis.