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Gastric colonization with Candida albicans

R A Greenfield1, W A Joyce

  • 1Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.

Mycopathologia
|April 1, 1993
PubMed
Summary
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A new rat model allows studying Candida albicans gastric colonization. Steroid treatment increased fungal colonization, while acid suppression and antiserum did not inhibit it.

Area of Science:

  • Mycology
  • Infectious Diseases
  • Gastroenterology

Background:

  • Candida albicans is an opportunistic pathogen that can cause gastrointestinal infections.
  • Understanding the factors influencing gastric colonization by C. albicans is crucial for developing effective treatments.
  • Existing models for studying gastric candidiasis in vivo are limited.

Purpose of the Study:

  • To adapt a rat model for studying in vivo gastric colonization by Candida albicans.
  • To investigate the effects of host modifications on gastric colonization by C. albicans.
  • To assess the potential of an antiserum to inhibit gastric colonization by C. albicans.

Main Methods:

  • A rat model of gastrointestinal candidiasis was adapted using antibiotic pretreatment to establish stable gastric colonization.

Related Experiment Videos

  • Host modifications including induction of gastric erosions (HCl), glucocorticosteroid treatment, and acid neutralization (famotidine) were tested.
  • In vitro preincubation of C. albicans with a polyclonal antiserum was performed before inoculation.

    • Main Results:

    • Antibiotic pretreatment (chloramphenicol and gentamicin) enabled stable gastric colonization of C. albicans in rats for at least 5 days.
    • Glucocorticosteroid treatment significantly increased C. albicans gastric colonization compared to controls.
    • Neither dilute HCl to induce erosions nor famotidine to neutralize acidity significantly altered C. albicans gastric colonization.
    • Preincubation of C. albicans with antiserum did not inhibit gastric colonization in this model.

    Conclusions:

    • The adapted rat model is suitable for studying in vivo gastric colonization by C. albicans.
    • Glucocorticosteroids enhance gastric colonization by C. albicans, suggesting immune suppression plays a role.
    • Gastric acidity and superficial erosions may not be primary factors limiting colonization in this model.
    • The tested antiserum was ineffective in inhibiting C. albicans gastric colonization in vivo.